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Tomography 4.0 software

The fast, easy and complete solution for electron tomography

Cryo electron tomography is a technique for recording images at various alpha tilts and then back-projecting them into a 3D reconstruction. Crucial to obtaining 3D image information is retrieving 3D data from the specimen.  Tomography 4.0 makes collecting this data easy and efficient. 




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Cryo electron tomography is a well-established technique for recording images at various alpha tilts and subsequent back-projection into a 3D reconstruction. Crucial to obtaining 3D image information is application software that effectively and easily retrieves 3D data from the specimen under investigation.

 

Backprojection of individual 2D images taken at different tilt angles during a tomography experiment
Image courtesy of MPI Martinsried, Germany
Final reconstruction
Image courtesy of MPI Martinsried, Germany

Thermo Fisher Scientific's latest Tomography 4.0™ software provides a fast, easy and complete solution for electron tomography using our Titan™ or Tecnai™ transmission electron microscopes and our latest detector modules. It provides easy access to 3D information by adding automation to key elements of the tomography workflow. Also, adding on the fly reconstruction during the actual tomography experiment allows for on the fly visualization of the reconstructed volume.

With the unique Tomography 4.0 version, the output of tomographic reconstructions can be significantly improved to the conventional reconstruction throughput.

Features and benefits

  • On the fly reconstruction algorithm which allows quality assessment already during acquisition; for higher yield and reduced time to data.
  • Easy graphical setup for Low Dose specimens not only for single acquisition, but also in batch mode to minimize damage.
  • Fully automatic TEM and STEM acquisition of multiple signals for life science and materials science applications.
  • Easy navigation allows for a simple batch setup, defining all positions where tomography series will be taken automatically.
  • Grid atlas acquisition provides an overview of the entire specimen for fast navigation.
  • One time calibration fits all: Common calibrations between tomography and EPU enable easy time efficient operation.
  • Asy workflow from 3D data to information with Inspect 3D, EPU and tailored data mining with scripting

 

 

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