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Standing waves

Three beam interference lithography in negative tone photoresist on crystalline silicon substrate. Regular pattern in horizontal and vertical directions.

Courtesy of Dr. Tomas Tamulevicius , Kaunas University of Technology

Taken by Quanta SEM microscope

Magnification: 20,000x
Sample: Photoresit on silicon
Detector: SE
Voltage: 20 kV
Horizontal Field Width: 12.8um
Working Distance: 9.8
Spot: 3.5

Sweat Spherules

spherules of sweat

Courtesy of Dr. Antonietta Gatti , Nanodiagnostics

Taken by Quanta SEM microscope

Magnification: 7256
Voltage: 26.7
Vacuum: 0.61 Torr
Horizontal Field Width: 41.12
Working Distance: 10
Spot: 3.9

Package Level Circuit Edit

Package Level Circuit Edit. Large area Polyimide removal with Plasma FIB.

Courtesy of FEI

Taken by Vion Plasma microscope

Sample: silicon
Horizontal Field Width: 261 μm
Working Distance: 17.1 mm

Golden Joystick

Fused gold wire during an electrical stress

Courtesy of Marie Castignolles

Taken by Nova NanoSEM microscope

Sample: Silicon Die
Detector: SE
Voltage: 2kV
Horizontal Field Width: 150µm
Spot: 3

Stars Colloid

Hydrothermal synthesis of ZnO results in star shaped nano particles. The SEM sample was prepared by drop-casting onto a silicon substrate.

Courtesy of Ms. Rhiannon Clark , RMIT University

Taken by Nova NanoSEM microscope

Magnification: 50,000x
Voltage: 5 kV
Working Distance: 3.0mm
Spot: 3.5

Sodium Phosphotungstate

Crystallized negative stain salts are usually seen as a source of artifacts, but here a certain aesthetic value was readily apparent.

Courtesy of Holger Wille

Taken by Tecnai microscope

Magnification: 25,000x
Sample: Crystallized sodium phosphotungstate
Voltage: 80 kV

3D Package X-section

3D Package X-section, Helios G4 PFIB

Taken by Helios G4 PFIB microscope

Membrane Protein Into Liposomes

Reconstitution of a membrane protein into liposomes

Courtesy of Jennifer Wingard

Taken by Tecnai microscope

Sample: Protein, lipid

Lamella preparation

Preparation of a lamella by focused ion beam (FIB) milling, for later viewing with TEM. The material is a semiconductor integrated circuit (IC) chip with micro-structure-surface passivation film

Courtesy of Dr. Maria Carbajo , UNIVERSIDAD DE EXTREMADURA

Taken by Quanta 3D microscope

Magnification: 7000x
Sample: semiconductor integrated circuit
Detector: SE
Voltage: 15kV
Horizontal Field Width: 45 μm
Working Distance: 8.3mm
Spot: 5.5

Cu-Zn Neuron Network

Cu-Zn intermetallics after dealloying of brass

Courtesy of Mrs. Seda Oturak , Middle East Technical University

Taken by Nova NanoSEM microscope

Magnification: 8000x
Sample: Copper-zinc (brass)
Detector: SE
Working Distance: 4.0 mm
Spot: 3.5

Polystyrole Insulating Foam

Section through a Polystyrole insulating foam. Showing the fine Walls of the Material and polymere structure.

Courtesy of Oliver Meckes

Taken by Quanta SEM microscope

Magnification: 360:1x
Sample: Polymer
Detector: SE+BSE
Voltage: 7kv
Vacuum: High Vac
Horizontal Field Width: 410 µm
Working Distance: 9 mm

Ebola Virus Tomogram Overlay

Cryo TEM of Ebola virus, with tomogram overlay

Courtesy of Daniel Beniac

Taken by Tecnai microscope

Magnification: 80,000x
Detector: CCD
Voltage: 200 kV

Copper Creeping

Typical appearance of sulphides and bornite/djurleite particle exhibiting typical Widmannstatten texture.

Courtesy of Musarrat Safi

Taken by MLA microscope

Magnification: 1614x
Sample: typical copper nickel reaction
Detector: BSE
Voltage: 25 kV
Vacuum: -7 kbar
Horizontal Field Width: 150.
Working Distance: 11mm
Spot: 7.0

Trabecular Rete of Human Oculus

The trabecular rete of human oculus consists of crossing collagen fibers with cells and extracellular matrix on the surface. Also on the surface of the trabeculae we can see round granules (not bacteria) of organic origin. The use of SEM in ophthalmologic microsurgery allows to evaluate the quality of surgical technique. These studies were conducted jointly with Lyubimova T.S. from Scientific Technical Complex “Microsurgery of oculus”.

Courtesy of Natalia Shevlyagina

Taken by Quanta SEM microscope

Magnification: 8,000x
Detector: ETD
Voltage: 5 kV
Vacuum: 1,55E-3 Pa
Horizontal Field Width: 37,3μm
Working Distance: 7,9 mm
Spot: 2,5 nA

Purple Petal

Image of a flower petal; courtesy of student Sara Kuzmenka.

Courtesy of Alyssa Calabro

Taken by Quanta 3D microscope

Pep-1, cell penetrating peptide

This is replica of pep-1, cell penetrating peptide prepared by freeze fracture method.

Courtesy of Mr. Han Lee , University of Minnesota

Taken by Tecnai microscope

Magnification: 9,300x

Pseudomonas Aeruginosa Bacterial invasion

The in vivo interaction between a wild type P. aeruginosa biofilm, on a silicone implant, and the responding polymorphonuclear leukocytes. SEM visualization depicts the interaction at day 1 post insertion of the implant in peritoneal cavity of a mouse. The leukocytes (yellow) are killed by the bacteria (cyan), as seen from their damaged appearance, with obvious cavities in the membrane, after contact with the WT biofilm.

Courtesy of Michael Larsen

Taken by Quanta 3D microscope

Magnification: 15000x
Detector: ETD
Voltage: 5.00kV
Horizontal Field Width: 22.8 µm
Working Distance: 6.1
Spot: 4.5

20-Minute Cross-Sectioned Bump

An 80 um wide and 100 um tall bump cross-sectioned with Vion in 20 minutes.

Courtesy of Courtesy of Sematech

Taken by Vion Plasma microscope

Magnification: 2000x
Horizontal Field Width: 128 μm
Working Distance: 16.6 mm

TSV Array

A cleaning cross-section mill pattern of 40 x 20 μm is used to clean up the section view of the TSV array for closer inspection (44 nA, 20 minutes)

Courtesy of Fraunhofer-Munich

Taken by Vion Plasma microscope

Magnification: 3500 x
Sample: silicon
Horizontal Field Width: 73.1 μm
Working Distance: 16.5 mm

Water droplets on leaf

Water droplets on the upper side of a leaf, showing the hydrophobic nature of the leaf surface

Courtesy of Dr. Jim Buckman , Heriot-Watt University

Taken by SEM microscope

Sample: Leaf and water
Detector: GSE
Voltage: 20 kV
Vacuum: ESEM mode 6.5 Torr
Horizontal Field Width: approx 400 microns
Working Distance: 8.4 mm
Spot: 5.2

Blood cell

Blood cell

Courtesy of Mr. MUHAMMET AYDIN , Namık kemal university

Taken by Quanta SEM microscope

Magnification: 200000
Sample: Blood cell
Detector: LFD
Voltage: 2
Vacuum: 86 Pa
Working Distance: 7,1
Spot: 3

Influenza Virus under EM

Influenza A virus sample taken via FEI Tecnai TF20, liquid ethane plunge frozen.

Courtesy of Mr. Long Gui , University of Washington

Taken by Tecnai microscope

Magnification: 11,500X
Sample: influenza A virus
Detector: CCD
Voltage: 200 kV
Vacuum: 6 mbar
Horizontal Field Width: 2.00
Working Distance: 4.0
Spot: 3.0

Nanofasern 10000xCol

Nano fibers after Ca-treatment

Courtesy of Mark Anderson

Taken by Quanta 3D microscope

Ice Cream

Ice Cream imaged on a Magellan XHR Scanning Electron Microscope (SEM)

Courtesy of FEI Image

Taken by Magellan XHR SEM microscope

Magnification: 200,000x
Detector: TLD
Voltage: 1.00 kV
Horizontal Field Width: 1.49 μm
Working Distance: 2.8 mm

MRSA Vs Macrophage

A mouse macrophage eating Staphylococcus aureus bacteria


Taken by Inspect microscope

Magnification: 12000
Sample: Cell and bacteria
Detector: EDT
Voltage: 25 kV
Horizontal Field Width: 24.9
Working Distance: 8.1 mm
Spot: 3.0